Anti-FosB, clone 4H90

The v-Fos oncogene was initially identified as the transforming gene of two independent murine osteosarcoma virus isolates (1,2) and an avian nephroblastoma virus (3). The cellular homolog, c-Fos, encodes a 62kD nuclear phosphoprotein that is rapidly and transiently induced by a variety of agents and functions as a transcriptional regulator for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers, which bind to specific DNA TPA response elements (TREs), c-Fos proteins are only active as heterodimers with members of the Jun gene family (4,5). Murine FosB encodes a nuclear protein of 338 amino acids which has 70% homology with c-Fos, exhibits similar kinetics of expression as c-Fos and forms heterodimers with both c-Jun and Jun B which bind to TRE DNA response elements (6). Functional homologs of c-Fos and FosB include Fra-1 and Fra-2 genes (7). Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 2ug/ml, Band at 46kD., Optimal dilutions to be determined by the researcher. Positive Control: HeLa cells, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.