Anti-FcgammaRI/CD64 Rabbit Monoclonal Antibody (Clone 027)

CD64, also known as high-affinity immunoglobulin gamma receptor I (FcgammaRI), is a transmembrane glycoprotein that binds the Fc region of IgG. It is composed of a ligand-binding alpha-chain, which contains three extracellular Ig-like domains, a transmembrane region, and a cytoplasmic domain, and two gamma-chains, which contain immunoreceptor tyrosine-based activation motifs (ITAMs) that mediate signal transduction. CD64 is constitutively expressed in myeloid cells, including monocytes and macrophages, but can be induced in neutrophils in response to IFN-gamma or G-CSF. It is involved in phagocytosis, cytokine release, antibody-dependent cell-mediated cytotoxicity (ADCC), and antigen presentation to primed T cells. Mouse CD64 selectively forms immune complexes with IgG2a, whereas human CD64 forms immune complexes with IgG1, IgG3, and IgG4. Knockout of Fcgr1, the gene encoding CD64, inhibits clearance of B. pertussis infection in mice. Increased serum levels of CD64 are associated with sepsis. Cayman's FcgammaRI/CD64 Rabbit Monoclonal Antibody (Clone 027) can be used for flow cytometry (FC), immunocytochemistry (ICC), and immunofluorescence (IF) applications.Synonyms: Fcgr1, Fc-gamma RIA, FcgammaRIa, FcRI, High Affinity IgGgamma Fc Receptor I, High Affinity Immunoglobulin Gamma Fc Receptor I, IgG Fc Receptor I. Immunogen: Recombinant mouse C-terminal His-tagged CD64 extracellular domain. Clone Designation: 027. Formulation: (Request formulation change), 50 or 100 µl of protein A-affinity purified monoclonal antibody. Storage Buffer: 0.2 µm filtered solution in PBS. Host: Rabbit. Isotype: IgG. Applications: FC, ICC, IF. Cross Reactivity: (+) CD64.