Anti-Factor VIII

Human Factor VIII is an essential blood coagulation factor. Whilst circulating in the blood, it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites, dissociates from the complex and interacts with Factor IXa, in the presence of calcium ions and phospholipids, to convert Factor X to the activated Factor Xa, which activates thrombin. Thrombin cleaves fibrinogen into fibrin, which polymerises and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause haemophilia A, a disorder characterised by the body's inability to control blood clotting. This could result in severe blood loss, even with minor injuries. Clone 7E127 is a very potent coagulation inhibitor. Applications: Suitable for use in ELISA, Western Blot and RIA. Other applications not tested. Recommended Dilution: ELISA: 1:500-1:15,000, Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-04D: IgG, H&L (AP) Pab Gt xMo, I1904-06F: IgG, H&L (FITC) Pab Gt xMo, I1904-06C: IgG, H&L (HRP) Pab Gt xMo, I1903-07E: IgG, (HRP) Pab Rb xMo, I1903-13A: IgG, (PE) Pab Gt xMo, Hybridoma: P3-NS/a-AG4-1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.