Anti-Ezrin, CT (Radixin, Moesin, Cytovillin, Villin-2, p81, VIL2, EZR)

Erzin, a member of the Ezrin, Radixin, Moesin (ERM) family, is a linker protein located between cell surface receptors, adhesion molecules, and actin cytoskeleton. Ezrin activity is regulated by intramolecular interactions between N- and C-terminal ERM association domains. Phosphorylation at threonine 567 is a critical regulator of Ezrin function allowing the active protein to link target molecules to the actin cytoskeleton. Erzin tyrosine phosphorylation can also be induced by EGF, PDGF and HGF stimulation. Erzin interacts with PI3-K protein kinase A and Rho. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:20,000, Western Blot: 1:500-1:1000, Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.