Anti-Estrogen Receptor beta5 (ERb5)

Eestrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ERbeta (ESR2), shown to exist in at least five isoforms (ERB1-ERB5). Although only the ERB1 variant appears fully functional, the remaining isoforms have been shown to form heterodimers with ERB1, thereby enhancing transcription., Immunohistochemical studies have revealed a ubiquitous expression of ERB5 in normal colorectal tissue, and to be present in all primary colorectal carcinomas studied (1). Studies also revealed the nuclear expression of ERB5 in the majority of breast carcinomas tested, with a possible association with improved patient survival (2). Applications: Suitable for use in Western Blot. and Immunohistochemistry Other applications not tested. Recommended Dilution: Immunohistochemistry (Paraffin): Requires antigen retrieval using sodium citrate buffer pH 6.0 heat treatment, Optimal dilutions to be determined by the researcher. Positive Control: Normal colon or colon carcinoma, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.