Anti-Estrogen Receptor b1, b2 (ERb1, 2)

Mouse/rat ERb1 gene encodes a protein of 485aa with a calculated size of approx. 54kD (1). Compared to ERa, ERb1 is highly conserved in the DNA-binding domain (> 90% homology) and the C-terminal ligand binding domain (55% homology). The A/B domain, the hinge region and the F-domain are not conserved. Rat ERb1 is primarily expressed in prostate, ovary, lung, bladder, brain, uterus and testis. Most recently functional variants of ERb1, termed ERb2, have been cloned. ERb2 has an additional 18aa as a results of in-frame mutation within the ligand-binding domain. ERb2 is expressed in ovary, prostate, pituitary, brain and muscle. Variants of both ERb1 and ERb2 (ERb1d3, ERb2d3) were detected that show deletion of 39aa in the DNA-binding domain. Both ERb1 and ERb2 specifically bind to ER response element. ERa, b1 and ERb2 have been found to heterodimerize with each other. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:100,000. Use 50-100ng control peptide/well., Western Blot: 1:1000-1:5000. Detects a band at ~54kD., Optimal dilutions to be determined by the researcher. Control Peptide: E3565-17N: Estrogen Receptor b1, b2, Human (ERb1, 2) Control Peptide, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.