Anti-ESAM (Endothelial Cell-selective Adhesion Molecule, UNQ220/PRO246, W117m)

Applications:, Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:100-1:200, Western Blot: 1:100-1:400, Immunohistochemistry (frozen): 1:100-1:500 , Immunohistochemistry (Paraffin): 1:50-1:200, Immunocytochemistry: 1:100-1:500, Optimal dilutions to be determined by the researcher. AA Sequence: MGHHHHHHSGSEF-LQLHLPANRL QAVEGGEVVL PAWYTLHGEV SSSQPWEVPF VMWFFKQKEK EDQVLSYING VTTSKPGVSL VYSMPSRNLS LRLEGLQEKD SGPYSCSVNV QDKQGKSRGH SIKTLELNVL VPPAPPSCRL QGVPHVGANV TLSCQSPRSK PAVQYQWDRQ LPSFQTFFAP ALDVIRGSLS LTNLSSSMAG VYVCKAHNEV GTAQCNVTLE VSTGPGAAVV AGAVVGTLVG LGLLAGLVLL YHRRGKALEE PANDIKEDAI APRTLPWPKS SDTISKNGTL SSVTSARALR PPHGPPRPGA LTPTPSLSSQ ALPSPRLPTT DGAHPQPISP IPGGVSSSGL SRMGAVPVMV PAQSQAGSLV, Positive Control Included: 140116A: ESAM, Positive Control, Suppled as a recombinant protein (aa31-390) in 100mM Tris, pH 8.8, 2% SDS, 200mM sodium chloride, 0.01% BPB, 0.02% sodium azide, 50% glycerol., DAB: 10ul/well, ECL: 5ul/well, Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.