Anti-EPCAM for IHC / Epithelial cell adhesion molecule, clone MSVA-326R

Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide. Epithelial cell adhesion molecule (EPCAM), also known as CD326, is a transmembrane epithelial glycoprotein that functions in cell adhesion, proliferation, and signaling within epithelial tissues and carcinomas. EpCAM Antibody for IHC is optimized for detection of CD326 expression in formalin-fixed, paraffin-embedded tissues, supporting high-resolution evaluation of epithelial lineage and tumor-associated staining patterns in histological sections.EpCAM antibody, also referred to as CD326 antibody or TACSTD1 antibody, is widely used in immunohistochemistry as a robust epithelial marker with strong membranous localization. EpCAM is highly expressed in normal epithelial tissues including gastrointestinal mucosa, glandular epithelium, and ductal structures, while non-epithelial tissues such as muscle, connective tissue, and most neural tissues remain largely negative. This distinct distribution makes EpCAM Antibody for IHC particularly valuable for identifying epithelial cells and distinguishing epithelial-derived tumors from surrounding stromal or mesenchymal components.Clone MSVA-326R is a recombinant rabbit monoclonal antibody developed for consistent and high-affinity detection of EpCAM in FFPE samples. This clone produces strong, clean membranous staining with minimal non-specific background, supporting clear visualization of epithelial architecture across a wide range of tissues. In Tissue Microarray (TMA) analysis, EpCAM Antibody for IHC demonstrates highly reproducible staining across hundreds of cores, enabling direct comparison of expression patterns between normal and malignant tissues within the same experimental framework.In normal tissue TMAs, EpCAM expression is prominently observed in epithelial compartments such as intestinal mucosa, pancreatic ducts, prostate glands, bronchial epithelium, and other glandular tissues, with consistent membranous HRP-DAB brown staining. In contrast, tissues lacking epithelial components show little to no staining, reinforcing the specificity of CD326 as an epithelial lineage marker. These staining patterns align with established protein expression datasets, supporting reliable interpretation in tissue-based studies.In cancer tissue microarrays, EpCAM Antibody for IHC reveals strong and often diffuse membranous staining in a wide range of epithelial malignancies, including colorectal, gastric, pancreatic, breast, ovarian, and lung carcinomas. Tumor cores frequently demonstrate increased staining intensity compared to corresponding normal tissues, reflecting EpCAM's role in tumor proliferation, signaling, and epithelial differentiation. Importantly, stromal regions, immune infiltrates, and non-epithelial tumor components remain largely negative, providing clear contrast that aids in tumor identification and morphological assessment.The performance of clone MSVA-326R in TMA-based immunohistochemistry supports its use in high-throughput tissue screening, biomarker validation, and comparative oncology studies. EpCAM Antibody for IHC enables consistent detection across large sample cohorts, making it well suited for studies evaluating tumor heterogeneity, epithelial differentiation status, and cancer progression. Its strong, reproducible staining characteristics and compatibility with standard antigen retrieval protocols make it a reliable reagent for FFPE tissue analysis and tissue microarray applications.This antibody is part of a broader collection of IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.