Anti-Endophilin B1 (Bif-1, Endophilin B1/B2, SH3-domain GRB2-like Endophilin B1/Bax-interacting Fact

Endophilins are among the best known BAR (Bin/Amphiphysin/Rvs) domain proteins. The highly conserved protein dimerisation domain is typically involved in membrane dynamics. Endophilin­B1, also known as SH3GLB1 (Src­Homology 3 Domain­containing GRB2­like protein B1) and BIF­1, is a 40kD protein that belongs to the endophilin family of molecules. It is a cytoplasmic and Golgi membrane protein that is expressed in neurons, striated (skeletal and cardiac) muscle cells, and placenta. Using SH3GLB1 in yeast two­hybrid screens, a second protein, SH3GLB2 (endophilin B2), was identified as an interacting partner. Endophilin­B1 plays a role in cell homeostasis. In neurons, Endophilin­B1 is phosphorylated by Cdk5, inducing homodimerization and autophagosome formation. In addition, it appears to play a role in the maintenance of mitochondrial integrity. Endophilin­B1 appears to cycle on­and­off the outer mitochondrial membrane (OMM), contributing to OMM integrity. And after the initiation of endocytosis, it also directs EEA1+ TrkA­containing endosomes back into the cell membrane for reuse. Human Endophilin­B1 is 365 amino acids (aa) in length. It possesses an N­terminal lipid­binding BAR (Bin/Amphiphysin/Rvs) domain (aa27­261) that contains an internal coiled­coil region, and a C­terminal SH3 domain that binds Pro­rich sequences on potential ligands (309­363). There are at least three potential splice variants for Endophilin­B1. Two are 42kD and 43kD in size, and possess a 21 and 29aa insertion after aa190, respectively. A third potentially utilizes an alternative start site at aa101. Over aa33­189, human Endophilin­B1 shares 98% aa sequence identity with mouse Endophilin­B1. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml, Immunohistochemistry: 8­25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.