Anti-Dystrophin, Rod Domain, clone 2Q1101

Dystrophin is a member of the spectrin/alpha-actinin family of actin-binding, triple helix rod-containing proteins. It is absent or greatly reduced in individuals with the X-linked recessive Duchenne's muscular dystrophy disorder, as well as in mice with the mdx (murine muscular dystrophy) mutation. Applications: Suitable for use in Western Blot, Immunohistochemistry and EM Gold. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:250, Immunohistochemistry (Fresh frozen, unfixed tissue): Neat 1:20, Not recommended for use on paraffin embedded tissue., EM Gold: Undiluted 90 minute incubation at 25°C. Light fixation with 2% formaldehyde + 0.001% glutaraldehyde for 1 hour. Use 2.3M sucrose as cryoprotectant. , Optimal dilutions to be determined by the researcher. Expected Staining Pattern: Light microscopy: Continuous rim of labeling at the periphery of muscle fibers, Western Blot: Strong double bands at ~400kD and metabolites of lower molecular mass , E.M. Gold: Close to the cytoplasmic face of the plasma membrane. Positive Control: Snap frozen normal human or rat striated muscle. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.