Anti-DOCK8 / Dedicator of cytokinesis protein 8

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. The DOCK8 antibody targets Dedicator of cytokinesis protein 8, a large guanine nucleotide exchange factor encoded by the DOCK8 gene. Dedicator of cytokinesis protein 8 regulates cytoskeletal dynamics by activating small GTPases of the Rho family, particularly CDC42, thereby controlling immune cell morphology, migration, and synapse formation. The DOCK8 antibody provides an essential tool for studying immune signaling, actin remodeling, and primary immunodeficiency syndromes associated with DOCK8 dysfunction.Dedicator of cytokinesis protein 8 contains a DOCK homology region (DHR) that mediates binding to CDC42 and phosphatidylinositol lipids. This structural configuration facilitates the localized activation of actin polymerization at the immunological synapse. The DOCK8 antibody enables visualization of this process, revealing how immune cells such as T cells, B cells, and natural killer cells use DOCK8 to maintain polarity and movement. Loss of function in DOCK8 disrupts actin organization, impairing immune synapse stability and antigen-specific responses.Mutations in the DOCK8 gene cause autosomal recessive hyper-immunoglobulin E syndrome (HIES), characterized by recurrent viral infections, elevated IgE levels, and defective T and B cell function. The DOCK8 antibody supports studies into this condition by allowing detection of protein expression in immune cell subsets from affected patients. Functional loss of Dedicator of cytokinesis protein 8 leads to increased susceptibility to viral skin infections and defective immune surveillance, highlighting its critical role in immune defense.Beyond immunodeficiency, Dedicator of cytokinesis protein 8 has been implicated in cancer biology, where its dysregulation can influence metastasis and tumor cell migration. The DOCK8 antibody supports investigations into these broader roles by enabling quantification of expression in cancer cell lines and tissues. Through regulation of actin assembly and cell adhesion, DOCK8 contributes to both immune and non-immune cell motility control.The DOCK8 antibody performs effectively in western blotting, immunofluorescence, and flow cytometry, showing strong cytoplasmic localization consistent with its role in actin remodeling. NSJ Bioreagents provides this antibody as a validated, high-specificity reagent for immunology, cell signaling, and disease research. By enabling precise detection of Dedicator of cytokinesis protein 8, the DOCK8 antibody supports exploration of immune synapse dynamics, cytoskeletal regulation, and primary immunodeficiency mechanisms.