Anti-DMPO Nitrone Adduct Antiserum

DMPO is a spin trap that reacts with oxygen-, nitrogen-, sulfur-, and carbon-centered radicals. It also reacts with DNA base- and amino acid-derived radicals to form DNA- and protein-DMPO nitroxone adducts, respectively, which rapidly decay to form DMPO-nitrone adducts. DMPO-nitrone adducts were previously detected using electron paramagnetic resonance (EPR), however, immunochemical detection allows a higher level of sensitivity and throughput with greatly reduced sample consumption. Cayman's DMPO Nitrone Adduct Polyclonal Antiserum can be used for Western blot, immunocytochemistry, and ELISA applications. The antiserum recognizes various DMPO-nitrone adducts, including DNA and protein species.Synonyms: 5,5-Dimethyl-1-Pyrroline-N-Oxide Nitrone Adduct. Immunogen: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline N-oxide coupled to ovalbumin. Formulation: (Request formulation change), 100 µl of rabbit anti-DMPO nitrone adduct polyclonal antiserum. Host: Rabbit. Applications: ELISA, ICC, and WB. Origin: Animal/Rabbit.