Anti-DMPO (5,5-dimethyl-1-pyrroline-N-oxide)

Free radicals are highly reactive and generally short-lived species. Hence, most studies of free radicals have been restricted to analysis of the end products formed by their interaction with cellular biomolecules. One technology that has permitted direct study of free radicals is electron spin resonance (ESR). For very short lived radicals, spin traps have been empolyed to generate longer lived radicals that are more amenable to ESR analysis. The most widely used of these spin traps is 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). The reaction of DMPO with a protein radical gives rise to a nitroxide radical which can be disproportionate to hydrozylamine and nitrone adducts. Of these species, only the nitrone adduct can be considered stable. It has been shown that our DMPO antibody will specifically react with radicals in protein. DMPO reagent can be employed for the immunochemical identifcation and characterization of nitrone adducts that result from the reaction of protein radicals with DMPO by immuno-spin trapping. , Applications: , Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:5,000 , ELISA: 1:5,000 to 1:10,000 , Immunohistochemistry: 1:1000 (subject to optimization) , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.