Anti-DFF45, Cleavage Site (Asp224) (ICAD, DNA Fragmentation Factor, Inhibitor of Caspase Activated D

Human DFF45 and its mouse homologue ICAD function in normal cells as chaperones for caspaseactivated deoxyribonuclease (DFF40 or CAD) during its synthesis (1). The association of DFF45 (or its isoform DFF35) with DFF40 inhibits the DNAse activity of the latter (1-4). In vitro, DFF45 has been shown to be the target of several caspases including caspase-3,-6,-7,-8 and granzyme B (3). In vivo, caspase-3 is believed to be the primary enzyme responsible for processing DFF45 and for release of its C-terminal fragment (3,5). The cleavage of DFF45 inactivates its inhibitory function on DFF40 and causes nuclear DNA degradation by DFF40, leading to cell death (6,7). Specificity/Sensitivity: Cleaved DFF45 (Asp224) Antibody detects the carboxy-terminal fragment of DFF45 resulting from cleavage at Asp224. This antibody does not recognize full length DFF45 or other cleavage products of DFF45. Recommended Dilution: Western Blot: 1:250 , Optimal dilutions to be determined by the researcher., , Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.