Anti-Desmin for IHC, clone MSVA-651M

Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide. Desmin (DES) is a muscle-specific intermediate filament protein that plays a critical role in maintaining the structural organization and mechanical integrity of muscle cells. It is expressed in skeletal, cardiac, and smooth muscle tissues, where it forms a cytoplasmic filament network linking contractile apparatus to cellular architecture. In immunohistochemistry, Desmin is characteristically detected as strong cytoplasmic staining within muscle cells, making it a cornerstone marker for identifying myogenic differentiation. Desmin Antibody for IHC is widely used in formalin-fixed, paraffin-embedded tissues to visualize muscle lineage cells and evaluate tumor origin in histopathological analysis. This antibody can be compared with our Desmin Antibody (clone DES/2960R) for detection of desmin as a muscle marker with validation supported by gene knockdown.Desmin antibody, also referred to as DES antibody or muscle intermediate filament antibody in the literature, recognizes a cytoplasmic protein with highly restricted expression in muscle tissues. This Desmin Antibody for IHC is specifically optimized for Tissue Microarray (TMA)-based immunohistochemistry, enabling standardized, high-throughput evaluation of staining patterns across large panels of normal and cancer tissues. In normal tissue TMAs, strong and consistent cytoplasmic HRP-DAB brown staining is observed in skeletal muscle fibers, cardiac myocytes, and smooth muscle layers of organs such as the gastrointestinal tract and blood vessels, while epithelial, lymphoid, and most non-muscle stromal compartments remain negative, providing excellent contrast for interpretation.In cancer tissue microarrays, Desmin expression is robustly detected in tumors of muscle origin, including leiomyomas, leiomyosarcomas, and rhabdomyosarcomas, where diffuse cytoplasmic staining highlights tumor cells with myogenic differentiation. This staining pattern is particularly valuable in distinguishing soft tissue tumors with muscle lineage from morphologically similar neoplasms lacking Desmin expression. In contrast, carcinomas and most non-myogenic tumors typically show absent staining, reinforcing the specificity of Desmin Antibody for IHC in differential diagnosis and tumor classification. The ability to evaluate these lineage-specific patterns across hundreds of TMA cores enables consistent comparison of staining intensity and distribution across diverse tumor types.Tissue Microarray (TMA) analysis provides a powerful framework for assessing DES expression under uniform staining conditions, demonstrating highly reproducible results with strong signal in muscle-derived tissues and tumors alongside minimal background in non-expressing cell populations. The performance of clone MSVA-651M in TMA-based IHC highlights its ability to generate clear, well-defined cytoplasmic staining across a wide spectrum of tissues, supporting its use in large-scale immunohistochemistry studies, biomarker validation, and comparative tissue profiling.This antibody targets Desmin in research applications requiring precise and interpretable immunohistochemical detection of muscle lineage markers in FFPE tissue sections, making it well suited for studies of myogenic differentiation, soft tissue tumor classification, and muscle-associated disease biology.This antibody is part of the Desmin antibody collection, where additional DES antibodies can be explored.This antibody is also part of a broader collection of IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.