Anti-DARC (CT) (Duffy Antigen/Chemokine Receptor, Duffy Antigen/Chemokine Receptor, Fy Glycoprotein

CD234 (DARC) is a non-specific receptor for a number of cytokines including IL-8, GRO, RANTES, MCP-1 and TARC. CD234 also functions as the receptor for the malaria parasites Plasmodium vivax and P. knowlesi. CD234 is also responsible for the Duffy blood group system where a substitution at position 42 of either Gly-42 or Asp-42 results in Fy(A) or Fy(B). CD234 is present in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along post capillary venules throughout the body, except in the adult liver. Erythroid cells and post capillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium. It has been demonstrated that CD234 exists in multiple isoforms. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not tested. Recommended Dilution: Western Blot: 1-2ug/ml, Immunohistochemistry (Paraffin): 2.5ug/ml Requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer pH 6.0 is recommended. , Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies Sheep Anti Rabbit IgG HRP, Immunohistochemistry Positive Control Tissue: Mouse brain, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.