Anti-D6PK

Make to order. Production time: 70-80 business days. Deliverables: 1. 200 µg antigen (used as positive control), 2. 1 ml pre-immune serum (used as negative control) , 3. Rabbit polyclonal antibody, antigen affinity purified. Quality Guarantee: 1. Antibody purity > 90% confirmed by SDS-PAGE, 2. Antibody titer > 1: 64,000 confirmed by ELISA, 3. WB positive with the immunogen protein (not applicable for synthetic peptide). Applications: ELISA, WB (positive for the recombinant immunogen protein/peptide). Buffer: Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4. Protein function: Protein kinase that regulates the auxin transport activity of PIN auxin efflux facilitators by direct phosphorylation. D6PK-mediated PIN phosphorylation promotes auxin transport in the hypocotyl and this is a prerequisite for PHOT1-dependent hypocotyl bending. Phosphorylates PIN1, PIN2, PIN3, PIN4 and PIN7 in vitro and PIN1 in vivo. Phosphorylates PIN proteins (e.g. PIN1 and PIN2), especially when NPY proteins (e.g. NPY1/MAB4 and NPY5/MEL1) are recruited at the plasma membrane, this enhances the polarized localizations (apical or basal) of PINs in the cell by limiting their lateral diffusion-based escape (PubMed:33705718). Acts as a lipid domain-dependent mediator of root epidermal planar polarity via the binding to phosphatidylinositol phosphates (e.g. PtdIns(3)P, PtdIns(4)P, PtdIns(5)P, PtdIns(3,5)P(2), PtdIns(4,5)P(2), PtdIns(3,4,5)P(3) and phosphatidic acid) (PubMed:27251533). [The UniProt Consortium]