Anti-Cytokeratin, pan (Pan Keratin) (BSA & Azide Free)

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5 , CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing ce lls in epithelia during normal development and during the development of epithelial neoplasms. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml for 2 hours at RT, Flow Cytometry: 0.5-1ug/10e6 cells, Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissues sections in 10mM sodium citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. , Optimal dilutions to be determined by the researcher. Positive Control: Skin, Adeno- or Squamous carcinomas, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.