Anti-CYP7B1 / 25-hydroxycholesterol 7-alpha-hydroxylase

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. CYP7B1 antibody detects 25-hydroxycholesterol 7-alpha-hydroxylase, a cytochrome P450 enzyme that catalyzes the hydroxylation of oxysterols and steroids, contributing to bile acid synthesis and cholesterol homeostasis. Encoded by the CYP7B1 gene on chromosome 8q12.3, this microsomal monooxygenase regulates the alternative bile acid synthesis pathway by converting oxysterols such as 25-hydroxycholesterol and dehydroepiandrosterone (DHEA) into hydroxylated metabolites. CYP7B1 plays a key role in maintaining sterol balance and modulating neurosteroid and androgen metabolism.Localized to the endoplasmic reticulum membrane, CYP7B1 uses NADPH-cytochrome P450 reductase as an electron donor for hydroxylation reactions. It is highly expressed in liver, brain, and steroidogenic tissues, where it contributes to both cholesterol catabolism and neuroactive steroid metabolism. By regulating oxysterol levels, CYP7B1 influences liver lipid homeostasis and protects against cholesterol accumulation and neurotoxicity.The CYP7B1 antibody is widely used in metabolism, neurobiology, and endocrinology research to study bile acid biosynthesis, cholesterol turnover, and steroid regulation. Western blot analysis detects a 55 kilodalton band corresponding to CYP7B1, while immunofluorescence reveals endoplasmic reticulum localization in hepatocytes and neurons. This antibody provides a means to assess CYP7B1 expression under physiological and pathological conditions, including liver disease and metabolic disorders.Mutations in CYP7B1 cause hereditary spastic paraplegia type 5A and neonatal cholestasis, demonstrating the enzyme's essential role in cholesterol detoxification and neuronal maintenance. Altered CYP7B1 expression has also been linked to atherosclerosis, Alzheimer's disease, and hormonal dysregulation. The CYP7B1 antibody supports research into cholesterol metabolism, neurosteroid synthesis, and disease pathophysiology. NSJ Bioreagents provides this antibody validated for its applications, ensuring reliable detection in metabolic and neurological studies.