Anti-Cyclophilin A (Cyclosporin A Binding Protein, Peptidyl Prolyl Cis Trans Isomerase A, PPIASE)

Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells. Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000-1:10,000 detects Cyclophilin A in RIPA lysates from nonstimulated A431 cells. A previous lot detected Cyclophilin A in RIPA lysates from rat lung cells. 20ug of A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C8850 (1:10,000). Proteins were visualized using a Gt xRb IgG (HRP)* and a chemiluminescence detection system., Optimal dilutions to be determined by the researcher. *Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb, I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb, I1904-46Q: IgG, H&L (HRP) Pab Gt xRb, Included Positive Antigen Control: A0001-50: Non stimulated A431 cell lysate, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.