Anti-CXCR7 (C-X-C Chemokine Receptor 7, Chemokine Orphan Receptor 1, RDC-1) (PE)

The G protein-coupled receptor RDC-1 belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96% and 86% aa sequence identity, respectively. Reports of mRNA levels and/or protein expression (as assessed using anti-CXCR7, clone 9C4) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding and receptor level analyses (as assessed using anti-CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted primarily to tumor cells, activated endothelial cells, and fetal liver cells. The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells using MCF-7 human breast cancer cell line., Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma cells with B cells from a mouse immunized with a plasmid encoding human CXCR7. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.