Anti-CX3CR1 (Beta Chemokine Receptor-like 1, CCRL1, Chemokine C-X3-C Motif Receptor 1, CX3C Chemokin

CX3CR1 is a 40kD member of the delta-group of rhodopsin GPCRs. It is expressed on astrocytes, microglia, macrophages, Th1 and Tc1 T cells, NK cells, mouse Gr1lo monocytes plus smooth muscle and mast cells. CX3CR1 mediates adhesion to fractalkine, promotes avid binding of integrins to their ligands, and extends the life of monocytes. Mouse CX3CR1 is a 7-transmembrane protein that is 354aa in length. It contains a 32aa N-terminal extracellular region that shows no glycosylation, and a 56aa C-terminal cytoplasmic domain. Over aa1-32, 91-104, 169-196 and 258-274 collectively, mouse CX3CR1 shares 96% and 77% aa identity with rat and human CX3CR1, respectively. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug labels 10e6 cells. Validated with M1 mouse myeloid leukemia cell line., Western Blot: 1ug/ml detected CX3CR1 in mouse heart tissue and human embryonic heart tissue., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.