Anti-Crystallin AB (Alpha crystallin B chain, CryAB, Alpha (B)-crystallin, Rosenthal fiber componet,

AlphaB Crystallin (CRYAB, also known as Crystallin alpha-B chain and HspB5) is a 22-23kD member of the HSP20 family of proteins. It has widespread expression in many tissues in addition to lens epithelium, where it noncovalently oligomerizes with CRY-alphaA to generate a transparent 350-1000kD alpha-Crystallin protein complex. Human CRYAB is 175aa in length. There is a alpha-Crystallin Hsp domain over aa66-149. Multiple posttranslational modifications may exist. The N-terminal Met and MetAspIleAlaIleHis sequence is occasionally cleaved. There is also phosphorylation at Ser45 and 59, potential O-GlcNAc modification at Thr158, 162 or 170, and acetylation at Lys92. There is an alternate start site at Met68 that may be accompanied by a 47aa substitution for aa109-175. Full-length human CRYAB is 54% aa identical to CRY-AlphaA, and 98% aa identical to mouse CRYAB. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml, Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-28C: IgG, H&L (HRP) Pab DkxGt, I1904-28G: IgG, H&L (HRP) (X-Adsorbed) Pab DkxGt, I1904-28K: IgG, H&L (HRP) Pab RbxGt, I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab RbxGt, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.