Anti-CRTAC1 (Cartilage Acidic Protein 1, ASPIC1, CEP-68, CEP68)

CRTAC1 (cartilage acidic protein 1, also CEP-68) is a 95-105kD member of a novel family of EGF domain-containing proteins. It is secreted by articular chondrocytes and may play a role in either cartilage matrix organization, or cell-matrix adhesion. Mature human CRTAC1 is 634 amino acids (aa) in length. It contains four FG-GAP (PheGly-GlyAlaPro) domains (aa46-437) and one EGF-like motif (aa559-605). Multiple splice forms exist. There are two alternate start sites at Met9 and Met211 that may be accompanied by a 39aa substitution for the C-terminal 55 aa, or an 84aa substitution for aa45-661. Over aa28-661, human CRTAC1 shares 91% aa identity with mouse CRTAC1. This form in mouse, however, is more equivalent to the human isoform that shows a C-terminal 39aa substitution. In this case, there is 95% aa identity between mouse and human. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.