Anti-CREB1 / cAMP response element-binding protein 1

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. CREB1 antibody detects cAMP response element-binding protein 1, a nuclear transcription factor that regulates gene expression in response to cAMP, calcium, and growth factor signaling. Encoded by the CREB1 gene on chromosome 2q34, this protein belongs to the CREB/ATF family of transcription factors and is a master regulator of cellular proliferation, metabolism, and neuronal plasticity. CREB1 binds to the cAMP response element (CRE) in DNA, recruiting transcriptional coactivators such as CBP and p300 to stimulate RNA polymerase II-mediated transcription.Structurally, CREB1 contains an N-terminal kinase-inducible domain (KID) that undergoes phosphorylation at Ser133 upon activation by protein kinase A (PKA), calcium/calmodulin-dependent protein kinases (CaMKs), or MAPK pathways. This modification enables high-affinity binding to CBP/p300 and transcriptional activation of CRE-dependent genes involved in survival, learning, and glucose homeostasis. The C-terminal basic leucine zipper (bZIP) domain mediates DNA binding and dimerization with other CREB/ATF proteins.The CREB1 antibody is widely used in signal transduction, neuroscience, and endocrinology research to study gene expression control, synaptic plasticity, and second messenger signaling. Western blot analysis detects a 43 kilodalton band corresponding to CREB1, while immunofluorescence reveals nuclear localization with enrichment at transcriptionally active chromatin. This antibody is also effective for assessing phosphorylation-dependent activation in response to hormones, neurotransmitters, and stress stimuli.CREB1 activity is crucial for long-term memory formation, neuronal survival, and metabolic regulation. Dysregulation of CREB1 signaling contributes to neurological disorders, depression, diabetes, and tumorigenesis. In cancers, persistent CREB1 activation promotes proliferation and angiogenesis through induction of pro-survival genes such as BCL2 and VEGF. The CREB1 antibody provides a versatile reagent for dissecting transcriptional networks downstream of cAMP and growth factor pathways. NSJ Bioreagents validates this antibody for its applications, ensuring reliable detection for research into transcriptional and signaling regulation.