Anti-CRACC (SLAMF7, SLAM family member 7, 19A, CSI, CD2-like receptor-activating cytotoxic cells, CD

CRACC, also known as CSI (CD2 subset I), is a type I transmembrane protein belonging to the CD2 subset of the Ig superfamily. CRACC is expressed on most NK cells and subsets of CD8 + cells, CD4 + cells and B cells. CRACC may play a role in the activation and effector function of T cells and NK cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Direct): 0.5-1ug/ml with the appropriate secondary reagents to detect mouse CRACC. The detection limit for rmCRACC is ~1ng/well. , Western Blot: 2ug/ml. Lysates were prepared from mouse thymus extract in reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. Use of this antibody under non-reducing conditions is not recommended. The blot was developed with 2ug/ml of C7906-72B and chemiluminescent detection substrate., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.