Anti-Connexin 43 (Cx43, Gap Junction Alpha 1 Protein, CxA-1, GJA1, GJAL, Gap junction 43kD heart pro

A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexins, through which materials of low molecular weight diffuse from one cell to a neighboring cell. Each connexin is composed of a hexamer of connexin proteins. Connexins are a multi-gene family of highly related proteins. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages, class I (beta) and class II (alpha), have been identified in mammals. Connexin-43, also known as Gap Junction Alpha-1 (GJA1), belongs to the class II (alpha) group of connexins and is expressed in the heart and fetal cochlea. Applications: Suitable for use in Immunohistochemistry, Western blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml , Immunohistochemistry (formalin fixed and paraffin): 1-2ug/ml. Antigen retrieval is required., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Dilute in PBS (pH 7.4). Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.