Anti-Complexin 2 (CPX II, Synaphin-1)

Complexin-2 (CPX II, also known as synaphin-1) is a 19kD, cytosolic protein that belongs to the complexin/synaphin family of molecules. It is expressed in excitatory neurons and serves as a block against premature granule release by its binding to the cis-SNARE complex that contains SNAP25, VAMP2 and STX1A. Human Complexin-2 is 134 amino acids (aa) in length and contains one coiled-coil domain (aa 28-84) that incorporates part of a SNARE complex interaction sequence (aa 41-97). There is one potential splice variant that shows a premature truncation after Glu114. Human Complexin-2 is identical in aa sequence to that for mouse and rat Complexin-2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml. Lysates from human, mouse and rat brain tissue were resolved by SDS-PAGE, transferred to Immobilon-P membrane and immunoblotted with 1ug/ml goat , anti-Complexin-2. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.