Anti-Complement Factor H-Related Protein 5 (FHR5)

Complement factor H­related protein 5 (FHR­5 or CFHR5) is a 65 kDa member of the factor H­related protein family. It is secreted by the liver and circulates either free or bound to HDL. It is unique among FHR proteins in that it associates with complexes that arise from complement activation. It promotes C3b cleavage and blocks C3 convertase activity. Human CFHR5 is 551 amino acids (aa) in length. It contains nine, 60 aa SUSHI­, or short consensus repeats, plus 18 intrachain disulfide bonds. SUSHI repeats 5­7 (aa 267­444) reportedly mediate CFHR5 binding to CRP and heparin. The complement interaction site(s) is unknown. Human CFHR5 shares 64% aa sequence identity with canine CFHR5.. Applications: Suitable for use in Western Blot, Immunoprecipitation and Direct ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml. C7850-88A under non-reducing conditions only., Immunoprecipitation: 25ug/ml. Conditioned cell culture medium spiked with C7850-88A, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.