Anti-Collagen XIII A1 (Col13a1, Collagen alpha-1(XIII) Chain)

Collagen XIII A1 is an 85-95kD protein in the type 2 transmembrane collagen family. Mature mouse Collagen XIII A1 consists of a 40aa cytoplasmic domain, a 19aa transmembrane segment, and a 692aa extracellular domain (ECD). The ECD contains three collagenous regions separated by shorter non-collagenous regions. Within comparable regions of the ECD, mouse Collagen XIII A1 shares 85% and 88% aa sequence identity with human and rat Collagen XIII A1, respectively. Mouse Collagen XIII A1 is extensively spliced, with some isoforms showing a tissue specific distribution. Collagen XIII A1 is widely expressed during development and in the adult. It localizes to intercellular adherens junctions and cell-matrix focal adhesions. Collagen XIII A1 assembles into disulfide-linked trimers, a process that is enhanced by proline hydroxylation. Trimerization involves triple helix formation within the collagenous domains, although portions of the non-collagenous regions can also form coiled coils. The ECD of trimeric Collagen XIII A1 is an extended rod-like structure with two flexible hinges that correspond to non-collagenous regions. Collagen XIII A1 clusters in cholesterol-rich domains on the plasma membrane, and it can be cleaved from the cell surface or intracellularly by a furin-like protease. Collagen XIII A1 binds the extracellular matrix molecules fibronectin, heparin, integrin A1 nidogen-2, perlecan. The shed ECD retains its ability to bind fibronectin and can interfere with matrix formation. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Immunohistochemistry: 5-15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.