Anti-Clusterin (Clusterin Precursor, CLU, AAG4, Apolipoprotein J, APOJ, Apo-J, CLI, Complement-assoc

Clusterin is an ubiquitously expressed protein of two 40-kD chains, alpha and beta, covalently joined by disulfide bonds. It exists in two forms, a pro-apoptotic form localized to the nucleus and a pro-survival secreted form. Both are thought to be involved in DNA repair and cell cycle regulation. The secreted form is thought to inhibit apoptotic signalling by BAX, Clusterin suppression can sensitise cells to chemotherapy. Clusterin is overexpressed in human prostate cancer, breast cancers and squamous cell carcinomas. Part of the SC5b-9 complex, clusterin prevents the binding of a C5b-C7 complex to the membrane of the target cell inhibiting the complement cascade. Clusterin acts as an extracellular chaperone inhibiting the formation of human lysozyme amyloid associated with Alzheimer's disease. Clusterin is also important in spermatogenesis and is secreted by Sertoli cells. Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry (frozen), and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. , , Positive Control: Kidney from post streptocial glomerulonephritis patients. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.