Anti-Citrullinated Vimentin (Clone 12G11) - HRP Conjugated

Vimentin is a cytoskeletal intermediate filament protein present in cells of mesenchymal origin, including leukocytes, endothelial cells, and smooth muscle cells. It is subject to citrullination by peptidyl arginine deiminase 2 (PAD2. Cay-10785) under high calcium concentrations, which can occur during macrophage apoptosis. Citrullinated vimentin has been shown to have a role in the production of anti-citrullinated protein antibodies (ACPAs). Serum levels of immune complexes targeting citrullinated vimentin are increased in patients with rheumatoid arthritis and positive for ACPAs. Serum and plasma levels of citrullinated vimentin are also increased in patients with lung cancer or idiopathic pulmonary fibrosis, respectively. Cayman's Citrullinated Vimentin Monoclonal Antibody (Clone 12G11) - HRP Conjugated can be used for Western blot (WB). The antibody recognizes citrullinated vimentin at 54 kDa from human samples. Immunogen: Synthetic peptide from human vimentin with citrulline at R144 and R146 (G146R mutation). Clone Designation: 12G11. MW: 54 kDa. Formulation (Request formulation change): 100 µg of HRP-conjugated protein G-purified monoclonal antibody. Storage Buffer: PBS, pH 7.2, with 50% glycerol and 0.05% ProClinTM 300 and proprietary stabilizers. Host: Mouse. Isotype: IgG1. Applications: WB. Cross Reactivity: (+) Citrullinated vimentin (-) Unmodified vimentin. UniProt Accession No: P08670. Storage: -20°C. Stability: > 2 years.