Anti-Citrullinated Histone H3 Immunoaffinity Resin

Histone H3 is a nuclear protein and a component of the nucleosome core that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications, including citrullination. Histone H3 is subject to citrullination of arginine residues by peptidyl arginine deiminase 4 (PAD4) at positions 2, 8, 17 and 26, and this citrullination blocks methylation of these residues by protein-arginine methyltransferase 4 (PRMT4) and induces transcriptional repression of target genes. Citrullinated histone H3 is a component of neutrophil extracellular traps (NETs) that acts as an autoantigen to induce production of anti-citrullinated protein antibodies associated with various diseases such as sepsis, multiple sclerosis, rheumatoid arthritis, and multiple myeloma. Cayman's Citrullinated Histone H3 Immunoaffinity Resin is designed for immunoprecipitation (IP) of citrullinated histone H3 from biological samples. This is an effective way to concentrate citrullinated histone H3 for subsequent detection and analysis by Western blot (WB). The citrullinated histone H3 immunoaffinity resin consists of Cayman's Histone H3 (Citrullinated R2 + R8 + R17) Monoclonal Antibody (Clone 11D3) (Cay-17939) coupled to Sepharose. Synonyms: Citrullinated Histone H3 Immunoaffinity Beads Citrullinated Histone H3 Immunoaffinity Sorbent. Clone Designation: 11D3. Formulation: 200 µl of sepharose coupled to a histone H3 (Citrullinated R2 + R8 + R17) monoclonal antibody (Clone 11D3). Storage Buffer: PBS, pH 7.2, with 0.02% sodium azide. Host: Mouse. Isotype: IgG1K. Applications: IP. Cross Reactivity: (+) Citrullinated histone H3. Species Reactivity: (+) Human. UniProt Accession No: P68431. Storage: 4°C. Stability: > 2 years.