Anti-CIT / Citron Rho-interacting kinase

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. CIT antibody detects Citron Rho-interacting kinase, a serine/threonine kinase that regulates cytokinesis and neuronal development. CIT functions downstream of Rho family GTPases to coordinate actomyosin contractility and midbody formation during cell division. The CIT antibody is used in cell cycle, cytoskeletal, and neurodevelopmental research to study contractile ring organization and cytokinetic abscission.CIT is encoded by the CIT gene located on human chromosome 12q23.1. The protein is approximately 230 kilodaltons and contains an N-terminal kinase domain, a coiled-coil region, a Rho-binding domain, and a C-terminal citron homology region with multiple protein-protein interaction motifs. CIT localizes to the midbody during cytokinesis and to dendritic spines in postmitotic neurons.The CIT antibody detects the 230 kilodalton protein in western blot analysis and reveals distinct midbody and dendritic staining patterns. CIT acts as a scaffold and kinase that phosphorylates substrates including myosin light chain and actin-regulatory proteins, coordinating contraction during cell division. Depletion of CIT disrupts cleavage furrow ingression and leads to multinucleation due to failed cytokinesis.In the nervous system, Citron kinase contributes to dendrite formation, axon stability, and synaptic maturation. Mutations in CIT cause microcephaly and neuronal migration disorders due to abnormal mitotic spindle dynamics and impaired cell cycle progression in neural progenitors. CIT also interacts with RhoA and other GTPases, integrating cytoskeletal signaling with cortical actin organization.CIT is a critical effector of Rho-mediated contractility and has been implicated in cancer cell proliferation and migration. NSJ Bioreagents provides a validated CIT antibody optimized for its applications, enabling detailed studies of cytokinesis, cytoskeletal remodeling, and neurodevelopmental regulation.