Anti-CHP2 / Calcineurin B homologous protein 2

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. CHP2 antibody detects Calcineurin B homologous protein 2 (also known as CHP2 or Calcium-binding protein P22), a calcium-binding regulatory protein that modulates sodium-hydrogen exchanger (NHE) activity and signal transduction. Encoded by the CHP2 gene on chromosome Xq26.1, this EF-hand protein shares strong sequence similarity with CHP1 and serves as a cofactor for NHE family members and calcineurin-like enzymes. CHP2 contains three EF-hand calcium-binding motifs that undergo conformational changes upon Ca2+ binding, allowing dynamic interactions with target proteins and membranes. Through its calcium-sensitive regulatory role, CHP2 influences intracellular pH control, cell proliferation, and migration.CHP2 is expressed predominantly in the heart, skeletal muscle, and certain cancers, including colon and pancreatic tumors. It binds directly to the cytoplasmic tail of sodium-hydrogen exchangers (NHE1, NHE2, NHE3) to stabilize and activate their function. Unlike CHP1, which is ubiquitously expressed, CHP2 expression is more tissue-specific and often upregulated in malignant cells. By regulating NHE activity, CHP2 contributes to pH homeostasis essential for tumor cell survival and motility. Overexpression of CHP2 has been linked to enhanced proliferation and metastatic potential through activation of MAPK and AKT signaling pathways.The CHP2 antibody is widely used in cell signaling and cancer metabolism research to study calcium-regulated ion transport and tumor biology. Western blotting typically detects a 22 kilodalton band corresponding to the full-length protein, while immunofluorescence shows cytoplasmic and membrane localization consistent with NHE association. Researchers employ the CHP2 antibody to evaluate calcium-dependent signaling events and to compare CHP isoform expression in normal versus transformed cells. Its use extends to studies on cardiac physiology, as CHP2 modulates Ca2+-sensitive processes and contributes to excitation-contraction coupling. NSJ Bioreagents offers this antibody validated for its applications, ensuring precise detection in human and model systems.