Anti-Chloramphenicol (Chloromycetin)

Chloramphenicol is a chlorine-containing antibiotic produced by Streptomyces venezuelae. It has a wide spectrum of activity against gram-positive and gram-negative cocci and bacilli (including anaerobes), Rickettsia, Mycoplasma and Chlamydia. It inhibits prokaryotic protein synthesis by attaching to the 50S ribosomal subunit. This inhibits peptidyltransferase, thereby preventing the formation of peptide bonds. It also inhibits protein synthesis in mitochondria which accounts for its toxic effects that cause aplastic anemia. Chloramphenicol has other adverse effects such as bone marrow depression and gray baby syndrome. It's use is limited to serious infections such as those where resistance to other antibiotics occurs. It is still widely used in the treatment of typhoid fever, meningitis and eye infections. Applications: Suitable for use in competitive ELISA. Other applications have not been tested. Recommended Dilution: Optimal dilution to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells hybridized with spleen cells from BALB/c mice. , Affinity Constant: Not determined, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.