Anti-Chloramphenicol Acetyltransferase (CAT)

Chloramphenicol Acetyltransferase (CAT) is suitable for use in cell extract assays. CAT, encoded by Tn9, catalyzes the transfer of an acetyl group from acetyl-CoA to the 3'-hydroxy position of chloramphenicol. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:500, Western Blot: 1:500, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.