Anti-Chemerin (TIG2)

Chemerin, also known as TIG2, is a distant member of the cystatin superfamily. The Chemerin precursor is cleaved by multiple serine proteases at various sites near the C-terminus. Mature active Chemerin is a 15-16kD heparin binding protein that induces immature dendritic cell and macrophage chemotaxis through interactions with ChemR23. Mature mouse Chemerin shares 63% and 73% aa sequence identity with human and rat Chemerin, respectively. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 2-8ug/ml, Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma cells with B cells obtained from a rat immunized with purified, E. coli-derived, recombinant mouse Chemerin (aa17-156). Storage and Stability: Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.