Anti-CEA for IHC / Carcinoembryonic Antigen, clone MSVA-465R

Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide. Carcinoembryonic antigen (CEA), encoded by the CEACAM5 gene and also known as CD66e, is a glycosylated cell surface protein of the carcinoembryonic antigen-related cell adhesion molecule family that is widely expressed in epithelial tissues. In immunohistochemistry, CEA is classically detected at the apical and luminal surfaces of glandular epithelial cells, where it contributes to cell adhesion and epithelial organization. CEA Antibody for IHC is extensively used to evaluate epithelial differentiation in formalin-fixed, paraffin-embedded tissues, with its distinct membranous and luminal staining pattern providing clear visualization of glandular architecture and epithelial lineage in FFPE sections.CEA antibody, also referred to as CEACAM5 antibody or CD66e antibody in the literature, recognizes an epithelial-associated glycoprotein that is frequently upregulated and redistributed in malignancy. This CEA Antibody for IHC is specifically optimized for Tissue Microarray (TMA)-based immunohistochemistry, enabling high-throughput assessment of staining patterns across large panels of normal and cancer tissues under standardized conditions. In normal tissue TMAs, staining is typically restricted to apical surfaces of epithelial cells in gastrointestinal tissues such as colon and stomach, while stromal, lymphoid, and mesenchymal compartments remain largely negative, providing a low-background context that enhances interpretation of tumor-associated staining.In cancer tissue microarrays, CEA expression is frequently increased and shows expanded membranous and cytoplasmic HRP-DAB brown staining in a broad range of epithelial malignancies, including colorectal, gastric, pancreatic, and lung adenocarcinomas. Unlike the confined apical staining observed in normal tissues, tumor cells often exhibit diffuse and circumferential staining patterns, reflecting altered polarity and overexpression. CEA Antibody for IHC enables clear visualization of these changes across TMA cores, supporting differentiation of malignant epithelial cells from non-neoplastic tissue and aiding in identification of tumor origin in metastatic samples.Tissue Microarray (TMA) analysis allows direct side-by-side comparison of CEA expression across hundreds of individual tissue cores, demonstrating consistent and reproducible tumor-associated staining patterns with minimal background in non-epithelial tissues. The performance of clone MSVA-465R in TMA-based IHC highlights its ability to generate strong, well-defined staining in positive tumor populations while maintaining specificity. The observed staining distribution aligns with established CEA expression data, including datasets such as the Human Protein Atlas, reinforcing confidence in its use for large-scale immunohistochemistry studies.This antibody targets carcinoembryonic antigen in research applications requiring precise and interpretable immunohistochemical detection of epithelial tumor marker expression in FFPE tissue sections, making it well suited for studies of tumor classification, epithelial differentiation, and cancer-associated antigen expression.This antibody is part of the CEA antibody collection, where additional CEACAM5 antibodies for immunohistochemistry can be explored.This antibody is also part of a broader collection of IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.