Anti-Cdh1 / E-cadherin

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. Cdh1 antibody detects E-cadherin, a calcium-dependent cell adhesion glycoprotein critical for epithelial cell-cell junctions and tissue integrity. The UniProt recommended name is Cadherin-1 (CDH1), also known as E-cadherin. This transmembrane protein mediates homophilic interactions between epithelial cells, maintaining polarity and barrier function across tissues.Functionally, E-cadherin antibody identifies a 882-amino-acid protein composed of five extracellular cadherin repeats, a transmembrane segment, and a cytoplasmic domain that binds catenins. Through its linkage to beta-catenin and the actin cytoskeleton, E-cadherin forms adherens junctions that regulate tissue morphogenesis and cell migration. Its adhesive strength depends on calcium ions that stabilize the extracellular cadherin domains.The CDH1 gene is located on chromosome 16q22.1 and is strongly expressed in epithelial tissues, including gastrointestinal tract, liver, mammary gland, and skin. E-cadherin serves as a hallmark of epithelial differentiation, while its downregulation signifies epithelial-to-mesenchymal transition (EMT), a key event in cancer metastasis and wound repair.Pathologically, loss or mutation of E-cadherin leads to disrupted cell adhesion, increased invasiveness, and hereditary diffuse gastric cancer. CDH1 promoter methylation and transcriptional repression by SNAIL or TWIST contribute to tumor progression. Research using E-cadherin antibody supports studies in cell adhesion, cancer metastasis, and epithelial polarity.Cdh1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect E-cadherin in tissues and cultured epithelial cells. NSJ Bioreagents provides high-quality CDH1 antibody reagents optimized for cell adhesion, cancer biology, and developmental studies.Structurally, E-cadherin forms cis and trans dimers that bridge adjacent cell membranes, with its cytoplasmic domain binding to catenins and actin filaments. This antibody facilitates detailed characterization of E-cadherin's role in maintaining epithelial integrity and suppressing invasion.