Anti-CD8a (Ly2, T cell Co-receptor, Leu2, T8), APC conjugated, clone 5K7

In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an alpha chain (CD8alpha/Lyt-2) and a beta chain (CD8beta/Lyt-3), and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the "suppressor/cytotoxic" subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8a without CD8b. It has been suggested that CD8+beta- T cells mature extrathymically, while development of the CD8alpha+beta+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. 1-8, Applications:  Flow cytometry 8, 9,  Immunohistochemistry (acetone-fixed frozen sections) 10,  Immunoprecipitation 8,  In vivo and in vitro depletion of CD8a+ cells 1, 4, 5,  Fractionation of CD8a+ T cells 10, Recommended Dilution: Flow Cytometry: < 0.2ug/10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.