Anti-CD62L (LECAM-1, L-Selectin, Leukocyte, Endothelial Cell Adhesion Molecule) (Biotin)

Human CD62L is as adhesion molecule found on most leukocytes and plays a primary role in mediating initial leukocyte interaction with activated vascular endothelium (1). Human CD62L has signal transduction functions where engagement leads to increased calcium levels and upregulated expression of certain integrins (2). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ug/ml, Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured human Raji cells were washed and incubated 45 minutes on ice with 80ul of C2415-15B at a concentration of 5ug/ml. , 2. Cells were washed two times and incubated with S7973-88P: Streptavidin (PE) , 3. Washed three times, fixed and analyzed by FACS., 4. Cells stained positive with a mean shift of 1.72 log10 fluorescent units when compared to I1904-83V1: IgG2a Negative Control Murine (Biotin) at a similar concentration., 5. Binding was blocked when cells were pre-incubated 10 minutes with 20ul of C2415-15A, CD62L at a concentration of 0.5mg/ml. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.