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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
C2413-21R.200 | 200 µl | - | - |
3 - 19 business days* |
796.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Human CD59 is a GPI anchored molecule that protects cells from complement-mediated lysis (1).... more
Product information "Anti-CD59 (MACIF, MIRL, Protectin) (PE)"
Human CD59 is a GPI anchored molecule that protects cells from complement-mediated lysis (1). Studies are progressing to determine the regulatory mechanism for CD59 expression (2). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (~80ul/test), Optimal dilutions to be determined by the researcher. FACS Protocol: 1. Five x 10e5 cultured HPB-MLT cells were washed and preincubated ~5 minutes with 20ul of 250mg/ml human IgG (to block nonspecific binding). , 2. After which they were incubated 45 minutes on ice with 80ul of C2413-21R at a dilution of 1:50. , 3. Cells were washed three times, fixed and analyzed using a BD FACS Calibur. , 4. Cells stained positive with a mean shift of 2.81 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. , 5. Binding was 92% blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml C2413-21N. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Keywords: | Anti-MIRL, Anti-CD59, Anti-MACIF, Anti-MIC11, Anti-HRF20, Anti-HRF-20, Anti-MAC-IP, Anti-Protectin, Anti-1F5 antigen, Anti-MEM43 antigen, Anti-CD59 glycoprotein, Anti-MAC-inhibitory protein, Anti-20 kDa homologous restriction factor |
Supplier: | United States Biological |
Supplier-Nr: | C2413-21R |
Properties
Application: | FC |
Antibody Type: | Monoclonal |
Clone: | 7H165 |
Conjugate: | Phycoerythrin |
Host: | Mouse |
Species reactivity: | human |
Immunogen: | Human K562 tumor cells |
Format: | Affinity Purified |
Database Information
KEGG ID : | K04008 | Matching products |
UniProt ID : | P13987 | Matching products |
Gene ID | GeneID 966 | Matching products |
Handling & Safety
Storage: | +4°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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