Anti-CD55 (DAF, Decay Accelerating Factor) (Biotin)

This antibody binds with a single chain 70kD glycoprotein and identified as CD55 (also known as decay accelerating factor, DAF). CD55 is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI- anchored) protein and inhibits autologous complement cascade activation. CD55 also serves as receptor for CD97, echovirus and Coxsackie B virus. CD55 is a useful marker for the diagnosis of paroxysmal nocturnal hemoglobinuria (PNH). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: <0.5ug/1x10e6 cells in 100ul of staining volume followed by a secondary florescent conjugated anti-mouse antibody, Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.