Anti-CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (PerCP/Cy5.5)

The CD45 antigen is present on all human leucocytes including lymphocytes, monocytes, granulocytes, eosinophils, and basophils in peripheral blood. The antigen has a role in signal transduction, modifying signals from other surface molecules. The CD45 antibody has been reported to react weakly with mature circulating erythrocytes and plateletsThe antibody recognizes the human leukocyte common antigen which is found on cells from spleen, lymph nodes, 83% bone marrow cells and granulocytes. The antigen recognized is a glycoprotein with a molecular weight of 190kD-215kD. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ul is sufficient for staining of 1 million cells in a 100ul staining volume or 100ul of whole blood, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. PerCP conjugates are sensitive to light.