Anti-CD44 (CD44 antigen, CD44R, CDw44, CDW44, CSPG8, ECMR-III, Epican, Extracellular matrix receptor

Human CD44 molecules appear to serve as lymphocyte adhesion molecules with numerous functions in extracellular matrix binding, cell migration, hemopoisis, and lymphocyte homing. Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured Molt-4 human tumor cells were washed and incubated 45 min on ice with 80ul of anti-CD44 antibody at a concentration of 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.75 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.