Anti-CD44 (CD 44, CDw44, ECM III, ECMRIII, Epican, Extracellular Matrix Receptor III, GP90 Lymphocyt

CD44 Iis a a 100kD glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and of the breast. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:25-1:100, 10ul labels 10e6 cells in 100ul, Immunohistochemistry (Paraffin): 1:100-1:200. Requires antigen retrieval using heat treatment with citrate buffer pH 6.0 prior to staining of paraffin sections., Immunohistochemistry: Frozen sections, Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo, I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo, I1904-14C: IgG, Fc (HRP) Pab Gt xMo, I1904-19C: IgG, F(ab')2 (HRP) Pab Gt xMo, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.