Anti-CD41 (Integrin alpha IIb chain, gpIIb-IIIa, Platelet Membrane gpIIb-IIIa Complex)

Integrin alpha2b,(ITGA2b) also known as CD41 and GPIIb, is a transmembrane glycoprotein that is expressed by megakaryocytes and platelets. It is cleaved into two disulfide-linked chains (114kD and 22kD) during transit through the Golgi. ITGA2b associates with integrin beta3 to form complexes that interact with fibrinogen, von Willebrand factor, fibronectin, and vitronectin. ITGA2b is required for platelet aggregation, and defects lead to disorders of coagulation. Within the extracellular domain, mouse ITGA2b shares 81% and 89% amino acid sequence identity with human and rat ITGA2b, respectively. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml. Detection limit is ~40ng/well., Western Blot: 1-2ug/ml. Detection is ~25ng/lane under non-reducing and reducing conditions using colorimetric detection. Chemiluminescent detection will increase sensitivity by 5-50 fold. , Optimal dilutions should be determined by individual researcher. Hybridoma: Mouse myeloma with B cells obtained from a rat immunized with purified, CHO-derived, rmITGA2B (aa 32-988: Accession # NP_034705). Storage and Stability: Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.