Anti-CD4 (L3T4) (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Recepto

The antigen L3T4 is expressed by the helper/inducer subset of murine T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines. Applications: Suitabe for use in FACS, Immunofluorescence, Depletion, Flow Cytometry and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Flow Cyotmetry: 1ug to stain 1x10e6 cells, Optimal dilutions to be determined by the researcher. Hybridoma: SP2/0 myeloma cells with spleen cells from rat. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.