Anti-CD3e (T-cell Surface Glycoprotein CD3 epsilon Chain, T-cell Surface Antigen T3/Leu-4 epsilon Ch

CD3e is a 20kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3e forms a TCR complex by associating with the CD3d, g and z chains, as well as the TCR a/b or g/d chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: <1ug/10e6 cells in 100ul volume, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.